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Figure 4 illustrates that in our research least one full-grade clinical score after stable every baby was its own control (the data ee recovery, indicated by weight gain and at measures the stress level in every baby | === least one full clinical score. before and after vaccination). For a number Without going into great detail, strong of days there was no stress level in proliferation to the PLP 178-191 peptide breathing, then comes day 0 when the : J | used to induce disease was detected as early oe t— Adi a—ti— vaccine was administered and one can see as day 4 after "immunisation", reaching a how the babies reacted day by day. Figure peak by days 9-11. At the time of remis- 4 represents two babies (baby one and baby |= ——— === | sion, days 15-16, a considerable decrease in three) and one can see the individual proliferative capacity of lymph node cells differences in response, since baby three was detected. IFN-gamma (interferon reacted within the first 24 hours, and also gamma) production followed the same pat- that the highest stress level occurred for Std 5 | - Solel tern; some variability was observed between baby one on days 5-6, while for baby three individual mice, but a relatively high con- it occurred on day 7, but this is to be Figure 1: This record of “alarms in a baby centration was measured during the first 11 expected since babies are individuals in over a period of five-and-a-half months, days, decreasing thereafter. The highest their own right. One must also take into from October 1987 to March 1988, reveals concentration of IFN-gamma was measured consideration that in statistics you always _ that the stress-induced breathing pattern did _ at the time of disease onset, on day 11. The have a slight spread of a day or two before NOt subside after 21 days following vaccine response to PLP 178-191 gradually waned or after the critical days. One can also ministration. and was lowest at day 17, which in almost rephrase it that nature does not necessarily all mice is a silent period of the disease. operate in a sudden, cut-off fashion but in a Days 22-25 were characterised by an building-up and tapering-off way. increase in IFN-gamma production again: Figure 4 also illustrates the individuality this is the time point which, in most mice, of stress response after the 16th day: baby precedes detectable relapse. three had a significant delayed reaction Equally interesting are Takacs et al.'s towards the 24th day, while baby one had immunological time dynamics from days 42 only a slightly increased stress level to 48, as established by our monitoring of towards the 24th day. stress response to vaccination in babies. These are the days with increased stress level in breathing and increased numbers of deaths after vaccination. The weight loss/weight gain dynamics accompanying the above immunological challenge is equally relevant to babies after vaccination. Leif's and my studies confirmed the sitter ‘ RA on wm imi Wp ip Underlying mechanisms of the cycle Immunological research unwittingly pro- vided another explanation for the observed and recorded slight differences in the daily TUN dynamics of maximum stress response. Takacs et al. (1997) studied the possible fy, underlying mechanisms of the cyclic pattern validity of Hans Selye's concept of non- of relapsing/remitting experimental allergic Figure 2: Record of breathing in the form specific (or general adaptation) stress encephalomyelitis (EAE). Their approach of histograms stacked up at an angle. syndrome as a characteristic but non- was to conduct a longitudinal study corre- specific response in mammals to any lating epitope recognition and cytokine pro- noxious substance or insult or injury of any duction by draining lymph node cells, kind (Selye, 1978). However, since our splenocytes and central nervous system |**** ee recording of breathing was done with a non- (CNS) infiltrating cells with disease during |= touch medical technology (Cotwatch had a relapsing and remitting EAE. Responses of |= A sensor pad positioned under the mattress lymph node cells and splenocytes were uni- | = and nothing was attached to the body of the formative with respect to epitope spread. |= ,| | monitored person or an animal), we could However, there were interesting day-by-day |= al ba ols | scale record longitudinally for long periods of dynamics as far as the time-course of T cell ——— time (hour-by-hour or day-by-day recording responses in lymph nodes was concerned. _ Sanat tonto of stress dynamics in breathing), while EAE was induced with 200 micrograms of | = Selye studied the dynamics of adreno- PLP (proeolipid protein) 139-151, PLP | 7 cortical activity and had to perform invasive 178-191 or MBP (myelin basic protein) |, sm Mla ia ' lh blood tests which limited the density of his 87-106, emulsified in IFA supplemented record. His research only demonstrated the with 200 micrograms of Mycobacterium oe dynamics of stress response in very general tuberculosis and M. butyricum 8:1 and given | =«= terms as an alarm reaction (48 hours after subcutaneously (s.c.) on days 0 and 7. the insult), a stage of resistance (an Immediately after this "immunisation" and | * undetermined number of days after the first 48 hours later, mice received 200 nanograms . : P , | 48 hours) and a stage of exhaustion (another of Bordetella pertussis toxin (intraperi- il , alia aes ahi a | alarm-like reaction) following the stage of toneally) in PBS (protein baseline serum). resistance (of undetermined duration) Relapse was defined as a weight loss and Figure 3: Record of breathing in the form | @Pproximately corresponding to the 16th clinical worsening was characterised by at of vertical bars. day. Our much more detailed recording of ee 30 + NEXUS www.nexusmagazine.com OCTOBER — NOVEMBER 2005