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bioRxiv preprint doi: https://doi.org/10.1101/2020.01.30.927871; this version posted January 31, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Seq ID Insert 1 Insert 2 Insert 3 Insert 4 foses wncaoz0) ATG 1 CPET@GGACCAATEGTACTAAGAGG 1 1GA1|\1 1 Ac ERMNNMMMEMNMMMMM T cca t]c 1cc 1 Sat TENTEMMmT Too 4c|c ac T REMET MAT TEM EENEGGEGGE—EccTAG fare) wncaoz0|ePiisi 404227 A TG TC TETGGGACCAATGGTACTAAGAGGTTTGAT/ATTACCACAAAAACAACAAAAGTTGGAT/CTCCTOGTGATTETTCTTCAGGTTGGAC|AG ACT CAGACTAATTCTCCTCGGCGGGCACG TAG Jworancozojerissiso2120 A TG TCTETGGGACCAATGGTACTAAGAGGTTTGATIATTACCACAAAAACAACAAAAGTTGGATICTCC TGGTGATTCTTCTTCAGGTTGGAC|AGAC TCAGACTAATTCTCCTCGGCGGGCACG TAG Junancorsjensi.403931 A TG TC TE TEGGACCAATGGTACTAAGAGGT TTA TAT TACEACAAAAACAACAAAAGT GGA T/CTCCTEGTGATTETTETTCAGGT TOG AC|AG ACT CAGACTAATTCTCCTCGGCGGGCACC TAC }wunanzor9) eri ATG TCTETGGGACCAATGGTACTAAGAGGTTTOAT|ATTACECACAAAAACAACAAAAGTTGGAT|C GTGATTETTETTCAGGTTGGAC|ACAC TCAGACTAATTCTCCTCOGCGGGCACG TAG Jworanzors|erisi_so3929 A TS TCT ETGGGACCAATGGTACTAAGAGGTTTGAT|ATTACCACAAAAACAACAAAAGT TOGA TIC TCC TGGTGATTCTTCTTCAGGTTGGAC|AGAC TEAGACTAATTCTCCTCGGCGGGCACG TAG ATG TCTETGGGACCAATGGTACTAAGAGGTTTGAT/ATTACEACAAAAACAACAAAAGTTGGAT/CTCCTGGTGATTCTTCTTCAGGTTGGAC|AG ACT CAGACTAATTCTCCTCGGCGGGCACG TAG fwuranzorsjerisiso2iso = ATG TC TET. fwuranzors|erisi_so2129 = A ToT CTE TS: WUMANROISIEPLSL402128 ATG TCTETGGGACCAATGGTACTAAGAGGTTTOAT/AT TAC CACAAAAACAACAAAAGTTGGAT/CTCCTOGTGATTCTTCTTCAGGTTOGAC|AGACTCAGAC TAM TCTCCTCGGCGGGCACC TAG WuMANROIs|ERLSL402127 A TG TCTETGGGACCAATGGTACTAAGAGGTT TOA T/A TT ACCACARAAACAACAAAAGTTGGAT|CTCCTGGTGATTCTTCTTCAGGTTGGACIAGACTCAGACTA WuManporsjerissi 402123 ATG TCTETGGGACCAATGGTACTAAGAGGTTTOA TAT TACCACAAAAACAACAAAAGTIGGAT|CTCCTGGTGATTCTTCTTCAGGTTGGAC|AG ce TECTCaGCGGGCACGTAG WuMANBOI9|ERLL 402124 A TG TC TETGGGACCAATGGTACTAAGAGGTT TOA TAT TAC EACAAAAACAACAAAAGTIGGAT/CTCCTGGTGATTCTTCTTCAGGT TOGA WuManporsjerissi_402123 ATG TCTETGGGACCAATGGTACTAAGAGGTTTOA TAT TACCACAAAAACAACAAAAGTIGGAT|CTCCTGGTGATTETTETTCA WUMANROISIEPLSL 402121 ATG TCTETGGGACCAATGGTACTAAGAGGTTTGA TAT TACCACAAAAACAACAAAAGTTGGAT|CTCC ac WUMANROISIEPLSL 402119 ATG TCTETGGGACCAATGGTACTAAGAGGTTTGAT|aTTACCACAAAAACAACAAAAGTTGGAT|CTCC act 1usq2020|€P1 st, 404233 ATG TC TETGGGACCAATGGTACTAAGAGGTTTGATa TT aC CACAAAAACAACAAAAGTTGGAT|CTCC acte NoNTHABURY2O20|€P1 51403903 4 To TC TETGGGACCAATOG TACTAAGAGGTTTGAT|A + +AC CACAAAAACAACAAAAGTTGGATICTCC NoNTHABUAIZO2O| EFL SL 402902 & TG TC TETGGGACCAATGGTACTAAGAGGTTTGATIA + +AC CACAAAAACAACAAAAGTTGGATICTCC \POCAMS-n.0572020) EPL SL_403924 TG TCT CTGGGACCAATGGTACTAAGAGGTTTGATIA TT ACCACAAAAACAACAAAAGT ‘GUANGOONG2020|€PLS1_403937 ATG TCTETGGGACCAATGGTACTAAGAGG TT TGA T/A 7 7A CCACAAAAACAACA ‘GuANGOONG2020|€P1 51403998 ATG TCTETEGGACCAATGGTACTAAGAGGTTTOAT ATT ACCACAAAAACAA, ‘GuaNG00NG2020|€PLI_ 403933 A TG TCTETEGGACCAATGGTACTAAGAGGTTTOAT|. 71a CCACAAAAACAAC ‘GUANGOONG2O20|€PLII_ 403994 ATG TCTETGGGACCAATGGTACTAAGAGGTTTOAT Ar ra ‘GUANGDONG2020| ELSI 403933 A TG TCTETGGGACCAATGGTACTAAGAGGTTTGAT|, 7 ‘GuaNeD0NG2020|€P1 51403932 A TG TCTETGGGACCAATGGTACTAAGAGGTT TGA BETACOWUSAWAI2020| FI SL, 40414 TG TC TETEGGACCAATGGTACTAAGAGGTTTS BATYUNNAN2O13] EPL'GL 402131 ATG TTTCAGCCGACCAATOGTATTAA ACCAATGGTACTAAGAGGTTTGAT|AT TAC CACAAAAACAACAAAAGTTGG41/CTCCTGGTGATTCTTCTTCAGGTTGGACIAGAC TEAGACTAATTC TCC TCGGCGGGCACS TAG ACEAATGGTACTAAGAGGTTTOAT|AT TAC EACAAAAACAACAAAAGT TOGA T/C TCCTEGTGATTETTETTCAGGTTOCACIAGAC TEAGACTAATTCTECTCGGEGGGEACS TAG TeTECTCGGCGGGCAcGTAG TECTEGGCEGGCACG TAG eeTEGEcGGacAcc tac eTceGcaascacctac cTeGGcaascacctac TeGGCGGGCACc TAG CTEABACTAATTCTCCTCGGCGGGCACC TAC CTEAGMETAATTCTCCTCGGCGGGCACE TAC CTEAGACTAATTCTCCTCGGCGGGCACG TAG TOGACIAGACTEAGACTAATTCTCCTCGGCGGGCACGTAG TOGAClAGACTEAGACTAATTCTCCTCGGCGGGCACG TAG Ac AC TEAGACTAATTCTCCTCGGCGGGCACSTAG Ac AC TEAGACTAATTETEETCGGCGGGCACGTAG TGATTCTTCTTCAGGTTOGAClAc Ac TEAGACTAATTETECTCGGCGGGCACG TAG GATTCTTETTCAGGTTOGAC Ac AcTEAGACTAATTCTCCTCGGCGGGCACGTAG GOTGATTETTETTCADB EOS AClac Ac TCAGACTAATTCTCCTCGGCGGGCACGTAG TOGATICTECTOSTOATTET ICY oA ClAGACTCAAACTAATIC- ~ACGTAG (2) Fig.S2: All four ins pre: 28 W ban 2015-nCoV virus genomes obtained e from GISAID. The gap in the B ke CoKig@ ast row shows that insert 1 and 4 is very unigue to Wuhan 2019-nCoV. Nels of \\S CAN et